ABSTRACT The objective of this study was to develop an injectable and biocompatible hydrogel which can efficiently deliver a nanocomplex of graphene oxide (GO) and vascular endothelial growth factor-165 (VEGF) pro-angiogenic gene for myocardial therapy. For the study, an efficient nonviral gene delivery system using
polyethylenimine (PEI) functionalized GO nanosheets (fGO) complexed with DNA VEGF was formulated and incorporated in the low-modulus methacrylated gelatin (GelMA) hydrogel to promote controlled and localized gene therapy. It was hypothesized that the fGOVEGF /GelMA nanocomposite hydrogels can efficiently transfect myocardial tissues and induce favorable therapeutic effects without invoking cytotoxic effects.
To evaluate this hypothesis, a a rat model with myocardial infarction was used, and the therapeutic hydrogels were injected intramyocardially in the peri-infarct regions. The secreted VEGF from in vitro transfected cardiomyocytes demonstrated profound mitotic activities on endothelial cells. A significant increase in myocardial capillary density at the injected peri-infarct region and reduction in scar area were noted in the infarcted hearts with fGOVEGF /GelMA treatment compared to infarcted hearts treated with untreated sham, GelMA and DNAVEGF /GelMA groups. Furthermore, the fGOVEGF /GelMA group showed significantly higher (p < 0.05, n = 7) cardiac performance in echocardiography compared
toothergroups,14dayspostinjection.Inaddition,no significantdifferenceswerenoticedbetweenGO/GelMAandnon-GOgroupsintheserumcytokinelevelsand quantitative PCR based inflammatory microRNA (miRNA) marker expressions at the injected sites. Collectively, the current findings suggest the feasibility of a combined hydrogel-based gene therapy system for ischemic heart diseases using nonviral hybrid complex of fGO and DNA.
The Pfizer document quoted in Expose article - “Structural and Biophysical Characterization of SARS-CoV2 Spike Glycoprotein (P2 S) as a vaccine antigen”- describes the use of graphene oxide as a technical tool for imaging and is not evidence that GO is an ingredient in the jabs, apparently. See recent substack by Sasha Latypova on this. However, she doesn't exclude the possibility that graphene oxide is utilized somewhere in Pfizer/Moderna manufacturing processes. See also comments section in Karen Kingston article you cross-posted a few hours ago where commenters Martin Chuzzle and D.K. Flynn point this out to her. She replies "It is an engineering technique so that there is an ionic molecular coupling of the proteins and GO. It is fairly new SBDD R&D technique. It’s my understanding this Sasha’s expertise. She should know better." She never misses an opportunity to take a swipe at Sasha Latypova. I'll try to watch the 2-hour video she did for Swedish group but frankly she is such a drama queen I doubt I'll get through it. Her constant biblical references and ostentatious background crosses also annoy me. She is after the limelight and happy to slander others to get it which is not how a true Christian behaves.
Yes, I read Sasha's piece. So the distinction is between whether the presence of graphene oxide is intentional or the result of poor manufacturing - not that it is not present in the injections or that it is toxic or that it could be in millions or billions of doses.
As she also pointed out, it is not the only possible source of Graphene Oxide.
"Expose-News.com also points to other potential sources of Graphene Oxide and why it might look yellowish, rather than brown or black-ish."
"One potential source of Graphene Oxide contamination could be the use of raw materials or reagents that contain the toxic substance or other graphene-based materials."
and
" if the equipment used in the manufacturing process is not properly cleaned or validated, this could lead to Graphene Oxide contamination of the vaccine."
and
"Another potential source of Graphene Oxide contamination could be through the use of filters or other materials used to sterilize or purify the vaccine."
So, I forced myself to listen to KK's interview. She also wrote about same in March substack article where there was some discussion as to whether or not this could be considered the smoking gun, incl. this comment from Marty Chuzzel: "Cryo Electron Microscopy is used for ultra-high resolution imaging for studies of protein structure. The gold foil and graphene oxide supports are specific to sample preparation for the microscopy and are not part of the process for making any vaccine components." The commenter also believes there's GO in the vaccines but that the document in question is not the smoking gun she claims it to be. So it seems she may be taking this out of context. In any case, she either doesn't reply to such comments or gives short shrift to their arguments. This needs to be sorted out between the experts who are unfortunately few and far between and many no doubt unwilling to delve into this.
Yes, I've followed all this quite closely and it could be either or as you say. Stefano Scoglio did a recent vid saying there's no GO in the shots. It's in Italian so I don't know how he comes to that conclusion. Stefan Lanka suggested some time ago it may come from composition of caps. To me, it makes perfect sense that it's in there deliberately. In any case, there doesn't seem to be proof the damage is being done by the spike protein either, or by that alone. Pharmacologist Maria Gutschi did a video a few months back (on team enigma's bitchute channel) where she says she's been searching for the metabolized spike protein for year a half - in vain. We absolutely need to get to the bottom of this and in the meantime, ban the shots altogether.
That's the one, first published by team enigma on 5 Nov 2022 - https://www.bitchute.com/video/ETNLgXbznTiH/ - minute 09:02: "I challenge anyone to show me a picture of the spike protein produced by the synthetic mRNA, available publicly. To the best of my knowledge, I cannot find it at all and I've spent a year and a half looking for it." Manufacturer's excuse was that it was proprietary information.
Given all the bad and nothing good about this “agent of death” vaccine...does anyone really believe the ever corrupt Progressives think that Biden or ANY other big wigs received this “vaccine”....not in a million years. America and the World have been conned...not just out of economic dollars and your future wealth but the very lives of your sons and daughters...parents and other loving relatives. Theirs were NOT natural in deaths and disability but created by humans to harm humans...mass elimination of human beings..inhumanity to man...this is criminal yet you hear little about prosecuting such and THAT reflects a fundamental shift in our entire construct as human beings..you let this go and there will be even more crimes and lost freedoms around the corner..just wait and see what these gangsters have in store for us next...they make the Mafia look like very small actors in the criminal underworld.
I wonder about that whole “did important people get saline” thing, but as deceptive as that is, that would also hopefully mean there is still some safety in being unvaccinated because if shedding was so toxic and prolific, the Big Guy interacts with many people and would be bound to be shed upon, so saline wouldn’t help him. Maybe he has a detox regimen us unimportants don’t have access to, but that’s not feasible to do 24/7.
Australian ex-federal gov't health minister Greg Hunt got very sick straight after the shot - cellulitis I think it was. Also our ex-Premier in Tasmania, Peter Gutwein, came down with chronic fatigue and collapsed in his office, later resigned. Then there was Gavin Newsome's alleged Bell's Palsy episode. So some of them got it. Unlikely the real top dogs though, as you say. Pity.
Seems to me that anyone talking about anything but the mRNA and instructions from that to make your cells start making spike protein gets ridiculed or ignored. Why is that?
Yes and also interesting is the silo between Hydrogel and GO. I imagine that with lot variability and corporate technology and DOD contracts for manufacture there are subsets of the injection technology. And why not? To perfect biowar this is a huge data set.
Nontheless why will Hydrogel and GO experts with their evidence not have a roundtable discussion online? I have followed Clifford Carnicom for 30 years. I have learned from La Quinta Columna for 3. Let's chat. The elephant exists. We blind men need not argue. In discussion truth arises.
Thank you PH. An excellent & in depth article, with profound implications for humanity's future.
Published online July 02, 2014 10.1021/nn5020787
C 2014 American Chemical Society
ABSTRACT The objective of this study was to develop an injectable and biocompatible hydrogel which can efficiently deliver a nanocomplex of graphene oxide (GO) and vascular endothelial growth factor-165 (VEGF) pro-angiogenic gene for myocardial therapy. For the study, an efficient nonviral gene delivery system using
polyethylenimine (PEI) functionalized GO nanosheets (fGO) complexed with DNA VEGF was formulated and incorporated in the low-modulus methacrylated gelatin (GelMA) hydrogel to promote controlled and localized gene therapy. It was hypothesized that the fGOVEGF /GelMA nanocomposite hydrogels can efficiently transfect myocardial tissues and induce favorable therapeutic effects without invoking cytotoxic effects.
To evaluate this hypothesis, a a rat model with myocardial infarction was used, and the therapeutic hydrogels were injected intramyocardially in the peri-infarct regions. The secreted VEGF from in vitro transfected cardiomyocytes demonstrated profound mitotic activities on endothelial cells. A significant increase in myocardial capillary density at the injected peri-infarct region and reduction in scar area were noted in the infarcted hearts with fGOVEGF /GelMA treatment compared to infarcted hearts treated with untreated sham, GelMA and DNAVEGF /GelMA groups. Furthermore, the fGOVEGF /GelMA group showed significantly higher (p < 0.05, n = 7) cardiac performance in echocardiography compared
toothergroups,14dayspostinjection.Inaddition,no significantdifferenceswerenoticedbetweenGO/GelMAandnon-GOgroupsintheserumcytokinelevelsand quantitative PCR based inflammatory microRNA (miRNA) marker expressions at the injected sites. Collectively, the current findings suggest the feasibility of a combined hydrogel-based gene therapy system for ischemic heart diseases using nonviral hybrid complex of fGO and DNA.
The Pfizer document quoted in Expose article - “Structural and Biophysical Characterization of SARS-CoV2 Spike Glycoprotein (P2 S) as a vaccine antigen”- describes the use of graphene oxide as a technical tool for imaging and is not evidence that GO is an ingredient in the jabs, apparently. See recent substack by Sasha Latypova on this. However, she doesn't exclude the possibility that graphene oxide is utilized somewhere in Pfizer/Moderna manufacturing processes. See also comments section in Karen Kingston article you cross-posted a few hours ago where commenters Martin Chuzzle and D.K. Flynn point this out to her. She replies "It is an engineering technique so that there is an ionic molecular coupling of the proteins and GO. It is fairly new SBDD R&D technique. It’s my understanding this Sasha’s expertise. She should know better." She never misses an opportunity to take a swipe at Sasha Latypova. I'll try to watch the 2-hour video she did for Swedish group but frankly she is such a drama queen I doubt I'll get through it. Her constant biblical references and ostentatious background crosses also annoy me. She is after the limelight and happy to slander others to get it which is not how a true Christian behaves.
Yes, I read Sasha's piece. So the distinction is between whether the presence of graphene oxide is intentional or the result of poor manufacturing - not that it is not present in the injections or that it is toxic or that it could be in millions or billions of doses.
As she also pointed out, it is not the only possible source of Graphene Oxide.
"Expose-News.com also points to other potential sources of Graphene Oxide and why it might look yellowish, rather than brown or black-ish."
The Expose-news.com article linked
https://expose-news.com/2023/04/09/pfizer-fda-lied-graphene-oxide-is-inside-covid-vaccines/
also has this:
"One potential source of Graphene Oxide contamination could be the use of raw materials or reagents that contain the toxic substance or other graphene-based materials."
and
" if the equipment used in the manufacturing process is not properly cleaned or validated, this could lead to Graphene Oxide contamination of the vaccine."
and
"Another potential source of Graphene Oxide contamination could be through the use of filters or other materials used to sterilize or purify the vaccine."
all this could be "weak sauce"
So, I forced myself to listen to KK's interview. She also wrote about same in March substack article where there was some discussion as to whether or not this could be considered the smoking gun, incl. this comment from Marty Chuzzel: "Cryo Electron Microscopy is used for ultra-high resolution imaging for studies of protein structure. The gold foil and graphene oxide supports are specific to sample preparation for the microscopy and are not part of the process for making any vaccine components." The commenter also believes there's GO in the vaccines but that the document in question is not the smoking gun she claims it to be. So it seems she may be taking this out of context. In any case, she either doesn't reply to such comments or gives short shrift to their arguments. This needs to be sorted out between the experts who are unfortunately few and far between and many no doubt unwilling to delve into this.
Yes, I've followed all this quite closely and it could be either or as you say. Stefano Scoglio did a recent vid saying there's no GO in the shots. It's in Italian so I don't know how he comes to that conclusion. Stefan Lanka suggested some time ago it may come from composition of caps. To me, it makes perfect sense that it's in there deliberately. In any case, there doesn't seem to be proof the damage is being done by the spike protein either, or by that alone. Pharmacologist Maria Gutschi did a video a few months back (on team enigma's bitchute channel) where she says she's been searching for the metabolized spike protein for year a half - in vain. We absolutely need to get to the bottom of this and in the meantime, ban the shots altogether.
https://www.bitchute.com/video/1MG7hHpprd9P/
That's the one, first published by team enigma on 5 Nov 2022 - https://www.bitchute.com/video/ETNLgXbznTiH/ - minute 09:02: "I challenge anyone to show me a picture of the spike protein produced by the synthetic mRNA, available publicly. To the best of my knowledge, I cannot find it at all and I've spent a year and a half looking for it." Manufacturer's excuse was that it was proprietary information.
Given all the bad and nothing good about this “agent of death” vaccine...does anyone really believe the ever corrupt Progressives think that Biden or ANY other big wigs received this “vaccine”....not in a million years. America and the World have been conned...not just out of economic dollars and your future wealth but the very lives of your sons and daughters...parents and other loving relatives. Theirs were NOT natural in deaths and disability but created by humans to harm humans...mass elimination of human beings..inhumanity to man...this is criminal yet you hear little about prosecuting such and THAT reflects a fundamental shift in our entire construct as human beings..you let this go and there will be even more crimes and lost freedoms around the corner..just wait and see what these gangsters have in store for us next...they make the Mafia look like very small actors in the criminal underworld.
I wonder about that whole “did important people get saline” thing, but as deceptive as that is, that would also hopefully mean there is still some safety in being unvaccinated because if shedding was so toxic and prolific, the Big Guy interacts with many people and would be bound to be shed upon, so saline wouldn’t help him. Maybe he has a detox regimen us unimportants don’t have access to, but that’s not feasible to do 24/7.
Australian ex-federal gov't health minister Greg Hunt got very sick straight after the shot - cellulitis I think it was. Also our ex-Premier in Tasmania, Peter Gutwein, came down with chronic fatigue and collapsed in his office, later resigned. Then there was Gavin Newsome's alleged Bell's Palsy episode. So some of them got it. Unlikely the real top dogs though, as you say. Pity.
Seems to me that anyone talking about anything but the mRNA and instructions from that to make your cells start making spike protein gets ridiculed or ignored. Why is that?
Yes and also interesting is the silo between Hydrogel and GO. I imagine that with lot variability and corporate technology and DOD contracts for manufacture there are subsets of the injection technology. And why not? To perfect biowar this is a huge data set.
Nontheless why will Hydrogel and GO experts with their evidence not have a roundtable discussion online? I have followed Clifford Carnicom for 30 years. I have learned from La Quinta Columna for 3. Let's chat. The elephant exists. We blind men need not argue. In discussion truth arises.
I totally agree with your comment. Where can I see Clifford's info?
Carnicom institute. Lots online.
"How many Red Flags are enough?"
Peter Nayland Kusk